Molecular, cytological and morphological studies on Jassid resistance in cotton (Gossypium hirsutum L.) based on hairiness trait

Subhashini, Selvaraj; Keerthivarman, Krishnan; Rajeswari, Sivakami; Premalatha, N; Kalaimagal, T; Muthuswami, M; Jeyakumar, P

doi:10.1186/s42397-025-00217-1

Table 1 Particulars of primer pairs used for molecular study

From: Molecular, cytological and morphological studies on Jassid resistance in cotton (Gossypium hirsutum L.) based on hairiness trait

S. No.	Marker name	Primer sequence (5’−3’)		Annealing temperature/˚C
1	BNL1153	F	CTTTATCCGGAGACGGAACA	55
1	BNL1153	R	CTAACTTTTGCTCACCCCCA	55
2	CIR316	F	TTACAGGCACTACCACC	60
2	CIR316	R	CCTTTCTGGCGACTT	60
3	BNL1122	F	TCGATAACGGCTATAGTAATCTCTC	55
3	BNL1122	R	CAACAAATAAGCAGCCAAGAAA	55
4	BNL3599	F	TTTAGCCCCAGTAACATGCC	55
4	BNL3599	R	ACTGCAAGCTCTGCCCTAAA	55
5	BNL3147	F	ATGGCTCTCTCTGAGCGTGT	55
5	BNL3147	R	CGGTTCAGAGGCTTTGTTGT	55
6	BNL1513	F	TTTCACAAGCACAACCATAGG	55
6	BNL1513	R	AATACAGGTTCAAAGTTGATAGGG	55
7	BNL1440	F	CCGAAATATACTTGTCATCTAAACG	55
7	BNL1440	R	CCCCCGGACTAATTTTTCAA	55
8	BNL1964	F	AGGGAGGGGGAGGTTCTC	55
8	BNL1964	R	CGGTAGTCCTCCACCATGTT	55
9	BNL1417	F	TTATTCTAACCACCGCCTCC	55
9	BNL1417	R	TGAGTGGATATGCTTGGCCT	55
10	CIR014	F	AGCTTGCCTCTTTCTG	60
10	CIR014	R	ACATTAGAACTCCCTGCT	60
11	BNL1878	F	TGCTTCAACTGCTCTTGCAT	55
11	BNL1878	R	TCGATATCTGGAACACCCAC	55
12	BNL2568	F	GGGAAGAGAGGGAGACTAACG	55
12	BNL2568	R	ATTTTGATAGGTTGGTTTTGTCC	55
13	BNL1059	F	CCTTCTCTGACACTCTGCCC	55
13	BNL1059	R	TGTATTCTCTTCTTTTCCTTATACTTTT	55
14	BNL1693	F	CCCTTGGGAATAGC	55
14	BNL1693	R	CATGTGTCTCCGTGTGTGTGTG	55
15	BNL2884	F	TCAACTCATACCAAATCAATTCC	55
15	BNL2884	R	CCCTGTTTTGTTCAATGGGT	55
16	NAU1215	F	GAGTGAGACTGGAGCTGGTT	55
16	NAU1215	R	CAAATCATTGTTTGCAGCAG	55
17	CIR199	F	CAGAATTTGACCGTTTC	50
17	CIR199	R	GCCATGATATTTCGGT	50
18	BNL3580	F	CTTGTTTACATTCCCTTCTTTATACC	55
18	BNL3580	R	CAAAGGCGAACTCTTCCAAA	55
19	MUSB1035	F	TCATGATAGCAACGAGTGGTGC	58
19	MUSB1035	R	GTCGAGTTGTATTAGTGTGCCCG	58
20	CIR0407	F	GCACAGAACATCCATACA	50
20	CIR0407	R	TCTCTCTCTCTTTCACACAC	50
21	BNL 1059	F	CCTTCTCTGACACTCTGCCC	55
21	BNL 1059	R	TGTATTCTCTTCTTTTCCTTATACTTTT	55
22	BNL 3255	F	GACAGTCAAACAGAACAGATATGC	60
22	BNL 3255	R	TGTATTCTCTTCTTTTCCTTATACTTTT	60
23	CIR 246	F	TTAGGGTTTAGTTGAATGG	55
23	CIR 246	R	ATGAACACACGCACG	55
24	CIR 307	F	GACTTGAAAAGATTACACAC	55
24	CIR 307	R	GAATTTGCTGGCTCT	55
25	JESPR 153	F	GATTACCTTCATAGGCCACTG	55
25	JESPR 153	R	GAAAACATGAGCATCCTGTG	55
26	JESPR 127	F	GATTTGGGTAACATTGGCTC	55
26	JESPR 127	R	CTGCAGTGTTGTGTTGGGTAGA	55
27	JESPR 154	F	GTTCCCTCAGTTGCTCAGAAG	55
27	JESPR 154	R	GGAGGAGTTGGCAGAAAATAGC	55

Back to article page

ISSN: 2523-3254

Contact us

Submission enquiries: pia.sustal@springernature.com
General enquiries: info@biomedcentral.com

Journal of Cotton Research

Contact us