Fig. 3

Principal component analysis (PCA) and Pearson correlation analysis heatmap of RNA-seq data. A PCA of samples at 5 development stages. SF1-1, SF1-2, and SF1-3, respectively, represent 3 biological replicates of SF1, and so on. Samples were clustered into 5 groups, respectively. SF1, SF2, SF3, SF4, and SFA represent the 1st, 2nd, 3rd, 4th instar nymphs and adult sexual females, respectively. B Pearson correlation analysis heatmap of 15 samples. In the heatmap, dark red represents a strong correlation, and blue denotes a weak correlation. Each column and row corresponds to the correlation between one sample and the other 14 samples (including itself)