Fig. 1

Effect of K+ deficiency on the transcriptional expression of GhHAK5aD in cotton. A The relative expression levels of GhHAK5aD under adequate (+K) and K+ low (-K) conditions in cotton cultivar CIM-554 in root and shoot. The expression levels in the root were normalized to 1. B, C K-inefficient (BH-212 and FH-901) and K-efficient (CIM-554, CYTO-124, and FH-142) cultivars at the three-leaf stage was transferred from adequate (+K) into K+ low (-K) solutions and grown for another 8 days. Root (B) and Shoot (C) samples were collected for qRT-PCR analyses. Bars represent mean ± SD (n = 3 biological replicates). Statistical analysis was performed using two-way analysis of variance (ANOVA) and Tukey’s post hoc test. Different letters denote significant differences (P < 0.05)